LOCUS       40924_26091        6449 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJG4-5 (pB42AD), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Gyuris J, Golemis E, Chertkov H, Brent R.
  TITLE     Cdi1, a human G1 and S phase protein phosphatase that associates 
            with Cdk2
  JOURNAL   Cell 75 (4), 791-803 (1993)
  PUBMED    8242750
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Gyuris J, Golemis E, Chertkov H, Brent R.
  TITLE     pB42AD (pJG4-5) complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6449)
  AUTHORS   Golemis E, Gyuris J, Chertkov H, Brent R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   4  (bases 1 to 6449)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6449)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1993"; volume: "75"; issue: "4"; pages: "791-803"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East Meadow Circle, 
            Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
            call (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor. For technical 
            information, call (415) 424-8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. If you suspect there is an 
            error in this sequence, please contact CLONTECH's Technical Support 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..6449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        81..522
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     CDS             546..566
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             573..809
                     /codon_start=1
                     /label=B42 transcriptional activator
                     /note="yeast transcriptional activator cretaed from E. coli
                     genomic DNA fragments (Ma and Ptashne, 1987)"
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
     CDS             816..842
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     terminator      1004..1191
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      1713..3055
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3419..4090)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(4091..4192)
                     /label=TRP1 promoter
     promoter        4295..4399
                     /label=AmpR promoter
     CDS             4400..5257
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5431..6019
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6307..6328
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6343..6373
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6381..6397
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6405..6421
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
       61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg
      121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
      181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
      241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
      301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
      361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
      421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
      481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaattaag cttatgggtg
      541 ctcctccaaa aaagaagaga aaggtagctg gtatcaataa agatatcgag gagtgcaatg
      601 ccatcattga gcagtttatc gactacctgc gcaccggaca ggagatgccg atggaaatgg
      661 cggatcaggc gattaacgtg gtgccgggca tgacgccgaa aaccattctt cacgccgggc
      721 cgccgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa gcggatgtta
      781 acgataccag cctcttgctg agtggagatg cctcctaccc ttatgatgtg ccagattatg
      841 cctctcccga attcggccga ctcgagaagc tttggacttc ttcgccagag gtttggtcaa
      901 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa
      961 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt
     1021 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     1081 ctcttaggtt ttaaaacgaa aattcttgtt cttgagtaac tctttcctgt aggtcaggtt
     1141 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccgag
     1201 caaatgcctg caaatcgctc cccatttcac ccaattgtag atatgctaac tccagcaatg
     1261 agttgatgaa tctcggtgtg tattttatgt cctcagagga caacacctgt tgtaatcgtt
     1321 cttccacacg gatcctctag agtcgactag cggccgcttc gacctgcagc aattctgaac
     1381 cagtcctaaa acgagtaaat aggaccggca attcttcaag caataaacag gaataccaat
     1441 tattaaaaga taacttagtc agatcgtaca ataaagcttt gaagaaaaat gcgccttatt
     1501 caatctttgc tataaaaaat ggcccaaaat ctcacattgg aagacatttg atgacctcat
     1561 ttctttcaat gaagggccta acggagttga ctaatgttgt gggaaattgg agcgataagc
     1621 gtgcttctgc cgtggccagg acaacgtata ctcatcagat aacagcaata cctgatcact
     1681 acttcgcact agtttctcgg tactatgcat atgatccaat atcaaaggaa atgatagcat
     1741 tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta aagggtagtg
     1801 ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag gtactagact
     1861 acctttcatc ctacataaat agacgcatat aagtacgcat ttaagcataa acacgcacta
     1921 tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa
     1981 cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg
     2041 ctttgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct
     2101 tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg
     2161 taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct
     2221 ttgctatata tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg
     2281 aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat tactctttag
     2341 acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa
     2401 acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac
     2461 caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg
     2521 tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta
     2581 atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa
     2641 gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta
     2701 tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct
     2761 cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct
     2821 ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     2881 gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata
     2941 gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     3001 ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgttaacaa
     3061 agatatgcta ttgaagtgca agatggaaac gcagaaaatg aaccggggat gcgacgtgca
     3121 agattaccta tgcaatagat gcaatagttt ctccaggaac cgaaatacat acattgtctt
     3181 ccgtaaagcg ctagactata tattattata caggttcaaa tatactatct gtttcaggga
     3241 aaactcccag gttcggatgt tcaaaattca atgatgggta acaagtacga tcgtaaatct
     3301 gtaaaacagt ttgtcggata ttaggctgta tctcctcaaa gcgtattcga atatcattga
     3361 gaagctgcag gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt
     3421 tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca
     3481 cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt
     3541 ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa
     3601 cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa
     3661 acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt
     3721 ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg
     3781 actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat
     3841 cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt
     3901 tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc
     3961 tattgggcac acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg
     4021 cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa
     4081 taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac gtgctcaata
     4141 gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa tttcttgaag
     4201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4621 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     4681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6121 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6181 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6241 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6301 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6361 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6421 catgattacg aattaattcg agctcggta
//
